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Top 10 PubMed Studies on GLP-1 Long-Term Safety (2026)

Last verified 2026-05-28 · 10 papers · every citation checked against PubMed

By Eli Marsden · Founding Editor
Editorially reviewed (not clinically reviewed) · How we verify contentLast reviewed

Most pivotal GLP-1 obesity trials run 68 to 72 weeks — long enough to register FDA approval, not long enough to answer the questions patients actually ask about ten-year safety. The papers below extend the safety window in two directions. Five are randomized trials with median follow-up between 2 and 5.4 years (STEP-5, SELECT, LEADER, SUSTAIN-6, REWIND), the longest-running prospective GLP-1 data in print. Two are obesity-trial extensions that capture what happens during continued dosing (SURMOUNT-4) or after withdrawal (STEP-1 extension). Three are large pharmacovigilance and observational studies — a JAMA cohort on gastrointestinal events, a FAERS disproportionality analysis of biliary signals, a WHO VigiBase replication on suicidality, and a multisite cohort on thyroid cancer — that probe rare adverse events the trials were never powered to detect. Older liraglutide post-marketing data extend further (10+ years), but the modern second-generation GLP-1 long-term record is still being written.

Ranked papers

#1STEP-5

Two-year effects of semaglutide in adults with overweight or obesity: the STEP 5 trial

Garvey WT, Batterham RL, Bhatta M, et al. · Nat Med · 2022

Primary endpoint: Body weight change and adverse-event profile at 104 weeks

STEP-5 extended the STEP-1 design to 104 weeks, the longest randomized obesity-trial follow-up for semaglutide 2.4 mg. Among 304 randomized adults, the safety profile at year 2 matched year 1: gastrointestinal adverse events remained the dominant category (82.2% semaglutide vs 53.9% placebo), most mild-to-moderate and concentrated during dose escalation. Serious adverse events occurred in 11.2% on semaglutide vs 6.6% on placebo. No new safety signals emerged in the second year of treatment, supporting the chronic-dosing paradigm but leaving longer follow-up to SELECT.

PMID 36216945NCT03693430

#2SELECT

Semaglutide and Cardiovascular Outcomes in Obesity without Diabetes

Lincoff AM, Brown-Frandsen K, Colhoun HM, et al. · N Engl J Med · 2023

Primary endpoint: First MACE and on-treatment serious adverse events over mean 39.8 months

SELECT randomized 17,604 adults with established cardiovascular disease and obesity (no diabetes) to semaglutide 2.4 mg or placebo with mean follow-up 39.8 months — the longest prospective safety dataset for a GLP-1 weight-loss dose. Serious adverse events were less common on semaglutide (33.4% vs 36.4%), but discontinuation for adverse events was higher (16.6% vs 8.2%), driven by gastrointestinal symptoms. Cholelithiasis was more frequent on semaglutide (2.8% vs 2.3%). No excess of pancreatitis, thyroid cancer, or psychiatric events was observed, providing the strongest medium-term obesity-dose safety signal to date.

PMID 37952131NCT03574597DOI 10.1056/NEJMoa2307563

#3LEADER

Liraglutide and Cardiovascular Outcomes in Type 2 Diabetes

Marso SP, Daniels GH, Brown-Frandsen K, et al. · N Engl J Med · 2016

Primary endpoint: First MACE and adjudicated adverse events over median 3.8 years

LEADER followed 9,340 type 2 diabetes patients on liraglutide 1.8 mg vs placebo for a median 3.8 years — the longest randomized safety record for any GLP-1 in modern use. Beyond the 13% relative MACE reduction, the safety dataset documented similar overall serious adverse-event rates (49.7% vs 50.4%), more acute gallstone disease (3.1% vs 1.9%), and no excess pancreatic cancer (0.3% vs 0.1%, p=0.06) or medullary thyroid carcinoma. Pancreatitis events were numerically lower on liraglutide. LEADER remains the canonical long-term GLP-1 safety dataset and continues to underwrite class-level FDA labeling.

PMID 27295427NCT01179048DOI 10.1056/NEJMoa1603827

#4SUSTAIN-6

Semaglutide and Cardiovascular Outcomes in Patients with Type 2 Diabetes

Marso SP, Bain SC, Consoli A, et al. · N Engl J Med · 2016

Primary endpoint: First MACE and adjudicated adverse events at 104 weeks

SUSTAIN-6 was the pre-approval cardiovascular safety trial for injectable semaglutide — 3,297 high-risk type 2 diabetes patients followed for 104 weeks on semaglutide 0.5 or 1.0 mg or placebo. Beyond a 26% MACE reduction, the safety dataset documented more retinopathy complications on semaglutide (3.0% vs 1.8%, HR 1.76, p=0.02), driven by rapid glycemic improvement in patients with pre-existing retinopathy. Discontinuation for gastrointestinal adverse events ran 11.5% (high dose) vs 5.7%. Neoplasm and pancreatitis rates were balanced. The retinopathy signal remains the most consequential long-term safety finding for semaglutide and shaped subsequent screening guidance.

PMID 27633186NCT01720446DOI 10.1056/NEJMoa1607141

#5REWIND

Dulaglutide and cardiovascular outcomes in type 2 diabetes (REWIND)

Gerstein HC, Colhoun HM, Dagenais GR, et al. · Lancet · 2019

Primary endpoint: First MACE and adjudicated adverse events over median 5.4 years

REWIND is the longest GLP-1 cardiovascular outcomes trial: 9,901 type 2 diabetes patients on dulaglutide 1.5 mg vs placebo for a median 5.4 years. With 69% enrolled for primary prevention, REWIND provides the deepest randomized safety dataset in a lower-risk population. Serious adverse-event rates were similar (45% vs 45%); pancreatitis, pancreatic cancer, and medullary thyroid carcinoma were all numerically balanced with no statistical excess. Discontinuation for gastrointestinal symptoms was 6%. REWIND extends the GLP-1 safety record past five years and remains the longest-duration randomized obesity-relevant safety dataset in print.

PMID 31189511NCT01394952DOI 10.1016/S0140-6736(19)31149-3

#6SURMOUNT-4

Continued Treatment With Tirzepatide for Maintenance of Weight Reduction in Adults With Obesity: The SURMOUNT-4 Randomized Clinical Trial

Aronne LJ, Sattar N, Horn DB, et al. · JAMA · 2024

Primary endpoint: Body-weight change and adverse-event profile from week 36 to week 88

SURMOUNT-4 captured 88 weeks of tirzepatide safety in 670 adults with obesity, including a 36-week open-label run-in plus 52-week randomized withdrawal. Discontinuing tirzepatide produced rapid weight regain (+14% over 52 weeks), while continued dosing remained well tolerated. Gastrointestinal adverse events during the maintenance phase were less frequent than in the SURMOUNT-1 escalation phase — most patients had completed dose escalation before randomization. Serious adverse events ran 4.7% on tirzepatide vs 5.0% placebo. SURMOUNT-4 is the longest randomized safety dataset published for tirzepatide as of 2026 and supports continuous rather than intermittent dosing.

PMID 38078870NCT04660643DOI 10.1001/jama.2023.24945

#7STEP-1 extension

Weight regain and cardiometabolic effects after withdrawal of semaglutide: The STEP 1 trial extension

Wilding JPH, Batterham RL, Davies M, et al. · Diabetes Obes Metab · 2022

Primary endpoint: Body weight and cardiometabolic risk factors one year after treatment withdrawal

This extension followed 327 STEP-1 completers for 52 weeks after stopping semaglutide 2.4 mg. Participants regained two-thirds of the lost weight (mean +6.9% vs the −17.3% lost during the trial) and most cardiometabolic improvements — blood pressure, lipids, A1C, hs-CRP — reverted toward baseline. The data are central to the long-term safety conversation because they quantify the consequence of discontinuation rather than continued dosing, anchoring the chronic-disease framing. No new adverse events emerged during the off-treatment phase. STEP-1 extension is the foundational withdrawal-physiology paper underpinning insurance coverage for indefinite GLP-1 use.

PMID 35441470NCT03548935

#8

Risk of Gastrointestinal Adverse Events Associated With Glucagon-Like Peptide-1 Receptor Agonists for Weight Loss

Sodhi M, Rezaeianzadeh R, Kezouh A, et al. · JAMA · 2023

Primary endpoint: Incidence of biliary disease, pancreatitis, bowel obstruction, gastroparesis

Sodhi and colleagues analyzed a PharMetrics Plus claims database of roughly 16 million U.S. patients to estimate post-marketing rates of serious gastrointestinal events on semaglutide or liraglutide vs bupropion-naltrexone for weight loss. GLP-1 use was associated with higher incidence of pancreatitis (HR 9.09), bowel obstruction (HR 4.22), and gastroparesis (HR 3.67), but not biliary disease. Absolute event rates remained low. The paper is the most-cited observational signal in the modern GLP-1 GI-safety conversation, and the gastroparesis finding drove the September 2023 FDA label update for ileus on Ozempic and Wegovy.

PMID 37796527DOI 10.1001/jama.2023.19574

#9WHO VigiBase replication

Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) and suicidality: A replication study using reports to the World Health Organization pharmacovigilance database

McIntyre RS, Mansur RB, Rosenblat JD, et al. · J Affect Disord · 2025

Primary endpoint: Disproportionality of suicidal ideation and behavior reports for GLP-1 vs comparator drugs

After early FAERS signals raised concern about GLP-1-associated suicidal ideation in 2023, McIntyre and colleagues replicated the analysis in the WHO VigiBase global pharmacovigilance database. The replication found no signal of disproportionate reporting for suicidal ideation or self-injury with semaglutide or liraglutide versus comparator anti-obesity and antidiabetic agents. Reporting odds ratios were below 1 for most pairwise comparisons. The paper provides important counter-evidence to the original 2023 FAERS report and supports the EMA's 2024 conclusion that no causal link could be established.

PMID 39433133

#10

Glucagon-Like Peptide 1 Receptor Agonists and Risk of Thyroid Cancer: An International Multisite Cohort Study

Baxter SM, Lund LC, Andersen JH, et al. · Thyroid · 2025

Primary endpoint: Incidence of thyroid cancer in GLP-1 vs DPP-4 inhibitor users across multiple national registries

Baxter and colleagues pooled Danish, Norwegian, and Swedish national health registries plus the U.S. Marketscan database to compare thyroid cancer incidence in GLP-1 receptor agonist users versus DPP-4 inhibitor users — a design that controls for diabetes severity. Across more than 200,000 GLP-1 users with mean 3.9-year follow-up, no excess thyroid cancer risk was observed (HR 0.93, 95% CI 0.66-1.31). Subgroup analyses by drug and dose were consistent. The study addresses the FDA boxed warning for medullary thyroid carcinoma carried over from rodent toxicology and provides the strongest population-level evidence that the warning may not translate to humans.

PMID 39772758

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We curate ranked, citation-anchored PubMed paper lists for the most-searched questions in obesity medicine. Every citation on this page was checked against PubMed on 2026-05-28. Each paper card links directly to PubMed and to ClinicalTrials.gov where applicable.

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